Thermostability
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Thermostability

 
 
 

Comparison of activities at different temperatures of commercial proteinases K and proteinase K manufactured by A&A Biotechnology

The assay was performed with 2 μg of each proteinase K at 25, 50 and 70°C, in 10 mM Tris-HCl pH 8.0 buffer at presence of 2% azo-casein as a substrate. The reaction was terminated by addition of 25% TCA, after 15 min.